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KMID : 0191120180330330238
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Journal of Korean Medical Science
2018 Volume.33 No. 33 p.238 ~ p.238
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Comparison of ELISA and Urine Microscopy for Diagnosis of Schistosoma haematobium Infection
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Song Hyun-Beom
Kim Ji-Young
Jin Yan
Lee Jin-Soo
Jeoung Hoo-Gn
Lee Young-Ha
Saeed Abd Al Wahab
Hong Sung-Tae
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Abstract
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Background: Schistosoma haematobium which causes urogenital schistosomiasis (UGS) is highly prevalent in African countries. Urine microscopy (UM) is the first-line diagnostic method of UGS. Enzyme-linked immunosorbent assay (ELISA) is a common method for screening many parasite infections primarily or alternatively. The present study established an in-house diagnostic system by ELISA and evaluated its diagnostic efficacy in comparison with UM for screening UGS in White Nile State, Republic of Sudan, 2011?2013.
Methods: A total of 490 participants were screened by UM or ELISA, and 149 by both. The in-house ELISA system was established employing soluble egg antigen of S. haematobium and the cut-off absorbance was set at 0.270.
Results: Of the 149 subjects, 58 participants (38.9%) were positive by UM, 119 (79.9%) were positive by ELISA and 82 (55.0%) showed consistently positive or negative results by both methods. The diagnostic sensitivity of ELISA was 94.8% and specificity was 29.7% based on UM results. The ELISA positive serum samples also cross-reacted with egg antigens of Schistosoma mansoni and Schistosoma japonicum.
Conclusion: We have established in-house ELISA for screening serum immunoglobulin (Ig) G antibodies by employing soluble egg antigen of S. haematobium for diagnosis of UGS with 94.8% sensitivity and 29.7% specificity. The ELISA system can supplement the conventional diagnosis by UM.
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KEYWORD
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Urogenital Schistosomiasis, Schistosoma haematobium, Diagnosis, Urine Microscopy, ELISA
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